Techniques / Tools

IMG_20150612_160615.jpg
IMG_20160915_214505.jpg
IMG_20150519_185423.jpg

In vivo imaging of layer 5 pyramidal neurons in the somatosensory cortex (labeled using the Thy1-GFP mouse

miniscopemodel.jpg
mouse417EZM_squareROI_threshold2.jpg
manycells.jpg

We are studying the role of immune genes in cortical synaptic development. During early postnatal development, some synapses are eliminated, in part, via microglia-dependent synaptic phagocytosis. We use a method called Expansion microscopy (ExM, from Ed Boyden's group) to visualize this biological process.

Expansionmicroscopy.tif

We can visualize synapses using FingRs (Fibronectin intrabodies generated with mRNA display), that bind endogenous neuronal proteins PSD-95 (Gross et al. 2013). Below are labeled synapses in a dendrite (Comer et al. 2020).

We can identify different neuronal subtypes using multiplex in situ hybridization (M-FISH, RNAScope). Here we stain for parvalbumin and somatostatin in the prefrontal cortex of a mouse

InsituPV_SST_PFC.png

We can dissect the connectivity patterns of moleculary identified brain cell types using simultaneous whole-cell patch-clmap recordings.